Protein Kinase A Regulatory Subunit II alpha Antibody (15G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26369
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 15G8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human PKA R2 [UniProt P13861]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protein Kinase A Regulatory Subunit II alpha Antibody (15G8)
Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] -
Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Image of Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.
Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] -
Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Image of Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.
Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] -
Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Staining of Hela with Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 512-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Protein Kinase A Regulatory Subunit II alpha Antibody (15G8)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PKA RII alpha
Long Name
cAMP-dependent Protein Kinase Regulatory Type II alpha
Alternate Names
PRKAR2A
Gene Symbol
PRKAR2A
Additional PKA RII alpha Products
Product Documents for Protein Kinase A Regulatory Subunit II alpha Antibody (15G8)
Product Specific Notices for Protein Kinase A Regulatory Subunit II alpha Antibody (15G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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