Protein Kinase A Regulatory Subunit II alpha Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03477

Novus Biologicals, part of Bio-Techne
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NBP3-03477-100ul
NBP3-03477-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKA RII alpha Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human Protein Kinase A Regulatory Subunit II alpha (NP_004148.1). MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Western Blot: Protein Kinase A Regulatory Subunit II alpha AntibodyAzide and BSA Free [NBP3-03477]

Western Blot: Protein Kinase A Regulatory Subunit II alpha AntibodyAzide and BSA Free [NBP3-03477]

Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody - Azide and BSA Free [NBP3-03477]

Immunocytochemistry/ Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody - Azide and BSA Free [NBP3-03477]

Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of L929 cells using [KO Validated] PRKAR2A Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Protein Kinase A Regulatory Subunit II alpha antibody
Western Blot: Protein Kinase A Regulatory Subunit II alpha AntibodyAzide and BSA Free [NBP3-03477]

Western Blot: Protein Kinase A Regulatory Subunit II alpha AntibodyAzide and BSA Free [NBP3-03477]

Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts of various cell lines, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA RII alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Long Name

cAMP-dependent Protein Kinase Regulatory Type II alpha

Alternate Names

PRKAR2A

Gene Symbol

PRKAR2A

Additional PKA RII alpha Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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