Protein Kinase D2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58315

Novus Biologicals, part of Bio-Techne
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NBP2-58315
NBP2-58315-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKD2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSS

Reactivity Notes

Mouse 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Kinase D2 Antibody

Western Blot: Protein Kinase D2 Antibody [NBP2-58315]

Western Blot: Protein Kinase D2 Antibody [NBP2-58315]

Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).
Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315]

Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315]

Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.

Applications for Protein Kinase D2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKD2

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Long Name

Protein Kinase D2

Alternate Names

nPKC-D2

Gene Symbol

PRKD2

Additional PRKD2 Products

Product Documents for Protein Kinase D2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein Kinase D2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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