Protein Phosphatase 1C gamma Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Protein Phosphatase 1C gamma Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Protein Phosphatase 1C gamma.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Protein Phosphatase 1C gamma Antibody
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP2-99488]
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP2-99488] - Anti-Protein Phosphatase 1C gamma rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDaImmunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody [NBP2-99488]
Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1C gamma Antibody [NBP2-99488] - Immunofluorescence staining of Protein Phosphatase 1C gamma in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Protein Phosphatase 1C gamma polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .Applications for Protein Phosphatase 1C gamma Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Protein Phosphatase 1C gamma
Alternate Names
EC 3.1.3.16, PP-1G, PP1gamma, PPP1G, protein phosphatase 1, catalytic subunit, gamma isoform, protein phosphatase 1, catalytic subunit, gamma isozyme, Protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Gene Symbol
PPP1CC
Additional Protein Phosphatase 1C gamma Products
Product Documents for Protein Phosphatase 1C gamma Antibody
Product Specific Notices for Protein Phosphatase 1C gamma Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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