Protein phosphatase 1F Antibody (6D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42648

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6D11

Format

BSA Free

Concentration

1 mg/ml

View all Protein phosphatase 1F Antibodies »

Product Summary for Protein phosphatase 1F Antibody (6D11) - BSA Free

Immunogen

Recombinant human Protein phosphatase 1F (1-454aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Protein phosphatase 1F Antibody (6D11) - BSA Free

Western Blot: Protein phosphatase 1F Antibody (6D11)BSA Free [NBP2-42648]

Western Blot: Protein phosphatase 1F Antibody (6D11)BSA Free [NBP2-42648]

Western Blot: Protein phosphatase 1F Antibody (6D11) [NBP2-42648] - The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for Protein phosphatase 1F Antibody (6D11) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein phosphatase 1F

Protein phosphatase 1F is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq]

Alternate Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaMKPaseCaM-kinase phosphatase, EC 3.1.3.16, FEM-2, hFEM-2, KIAA0015POPX2CAMKP, Partner of PIX 2, PP2C phosphatase, Protein fem-2 homolog, protein phosphatase 1F, protein phosphatase 1F (PP2C domain containing), protein phosphatase, Mg2+/Mn2+ dependent, 1F

Gene Symbol

PPM1F

UniProt

NP_055449 (Human)

Additional Protein phosphatase 1F Products

Product Documents for Protein phosphatase 1F Antibody (6D11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Protein phosphatase 1F Antibody (6D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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