Protocadherin-1 Antibody (5D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005097-M01
![ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-5D5-ELISA-H00005097-M01-img0011.jpg "ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PCDH1 (NP_002578 62 a.a. - 169 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFA
Localization
Cell junction. Cell membrane. Single-pass type I membrane protein. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries.
Specificity
PCDH1 - protocadherin 1 (cadherin-like 1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Protocadherin-1 Antibody (5D5)
![Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-5D5-Western-Blot-H00005097-M01-img0010.jpg "Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01]")
Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01]
Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01] - PCDH1 monoclonal antibody (M01), clone 5D5 Analysis of PCDH1 expression in A-431.![Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-5D5-Immunocytochemistry-Immunofluorescence-H00005097-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01]")
Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01]
Immunocytochemistry/Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on A-431 cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-5D5-Immunohistochemistry-Paraffin-H00005097-M01-img0008.jpg "Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01]")
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01]
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.Applications for Protocadherin-1 Antibody (5D5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Protocadherin-1
Alternate Names
PC42, PCDH1, PCDH42, Protocadherin1
Entrez Gene IDs
5097 (Human)
Gene Symbol
PCDH1
OMIM
603626 (Human)
UniProt
Additional Protocadherin-1 Products
Product Documents for Protocadherin-1 Antibody (5D5)
Product Specific Notices for Protocadherin-1 Antibody (5D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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