Protocadherin-12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77002

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Protocadherin-12 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12. The immunogen is located within amino acids 30 - 80 of PCDH12. Amino Acid Squence: KLSQELGREERRRQAGA

Specificity

This antibody is predicted to not cross-react with PCDH18.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protocadherin-12 Antibody - BSA Free

Western Blot: Protocadherin-12 AntibodyBSA Free [NBP1-77002]

Western Blot: Protocadherin-12 AntibodyBSA Free [NBP1-77002]

Western Blot: Protocadherin-12 Antibody [NBP1-77002] - Analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Protocadherin-12 Antibody - BSA Free

Immunohistochemistry: Protocadherin-12 Antibody - BSA Free [NBP1-77002] -

Immunohistochemistry: Protocadherin-12 Antibody - BSA Free [NBP1-77002] - Immunohistochemistry of Protocadherin-12 in rat lung tissue with Protocadherin-12 antibody at 5 ug/mL.

Applications for Protocadherin-12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protocadherin-12

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2,289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling. This antibody is predicted to not cross-react with PCDH18.

Alternate Names

PCDH12, Protocadherin12, VE-cad-2, VE-cadherin-2

Gene Symbol

PCDH12

UniProt

NP_057664 (Human)

Additional Protocadherin-12 Products

Product Documents for Protocadherin-12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Protocadherin-12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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