PRP18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92297

Novus Biologicals, part of Bio-Techne
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NBP1-92297
NBP1-92297-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRP18 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SNPVLELELAEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRKIEILTPEVNKGLRNDLKAALDKIDQQYLNEIVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRP18 Antibody

Western Blot: PRP18 Antibody [NBP1-92297]

Western Blot: PRP18 Antibody [NBP1-92297]

Western Blot: PRP18 Antibody [NBP1-92297] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PRP18 Antibody [NBP1-92297]

Immunohistochemistry-Paraffin: PRP18 Antibody [NBP1-92297]

Immunohistochemistry-Paraffin: PRP18 Antibody [NBP1-92297] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Western Blot: PRP18 Antibody [NBP1-92297]

Western Blot: PRP18 Antibody [NBP1-92297]

Western Blot: PRP18 Antibody [NBP1-92297] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Applications for PRP18 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRP18

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq]

Alternate Names

FLJ10210, hPrp18, pre-mRNA-splicing factor 18, PRP18, PRP18 homolog, PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae), PRP18 pre-mRNA processing factor 18 homolog (yeast), PRP18 pre-mRNA processing factor 18 homolog(PRPF18)

Gene Symbol

PRPF18

Additional PRP18 Products

Product Documents for PRP18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRP18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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