PRP19 Antibody (4M3Z1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16853
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4M3Z1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-504 of human PRPF19(Q9UMS4). FSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRP19 Antibody (4M3Z1)
Western Blot: PRP19 Antibody (4M3Z1) [NBP3-16853]
Western Blot: PRP19 Antibody (4M3Z1) [NBP3-16853] - Western blot analysis of extracts of various cell lines, using PRP19 Rabbit mAb (NBP3-16853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: PRP19 Antibody (4M3Z1) [NBP3-16853]
Immunocytochemistry/Immunofluorescence: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunofluorescence analysis of U-2 OS cells using PRP19 Rabbit mAb (NBP3-16853) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PRP19 Antibody (4M3Z1) [NBP3-16853]
Immunohistochemistry-Paraffin: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry of paraffin-embedded mouse brain using PRP19 Rabbit mAb (NBP3-16853) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for PRP19 Antibody (4M3Z1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRP19
Alternate Names
hPSO4, NMP200SNEV, Nuclear matrix protein 200, nuclear matrix protein NMP200 related to splicing factor PRP19, pre-mRNA-processing factor 19, PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), PRP19PRP19/PSO4 homolog (S. cerevisiae), PSO4PRP19/PSO4 homolog, Senescence evasion factor, UBOX4
Gene Symbol
PRPF19
Additional PRP19 Products
Product Documents for PRP19 Antibody (4M3Z1)
Product Specific Notices for PRP19 Antibody (4M3Z1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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