PrPC Antibody (3F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23335

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3F4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PrPC Antibodies »

Product Specifications

Immunogen

Clone 3F4 was generated from a synthetic peptide taken from amino acids in the central region of human prion protein. This sequence is conserved in feline and hamster, but is less conserved in rat and mouse PrPC. [UniProt# P04156]

Specificity

The antibody detects endogenous PrPC in human PC3 and A431 cell lines. In addition, the antibody strongly detects human recombinant Prion protein, and weakly detects bovine recombinant Prion protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PrPC Antibody (3F4)

PrPC Antibody (3F4)

Western Blot: PrPC Antibody (3F4) [NBP3-23335] -

Western Blot: PrPC Antibody (3F4) [NBP3-23335] - Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).

Applications for PrPC Antibody (3F4)

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PrPC

Prion diseases are transmissible neurodegenerative disorders which affect a range of mammalian species. In humans they can be inherited and sporadic as well as acquired by exposure to human prions. Prions appear to be composed principally of a conformational isomer of host-encoded prion protein and propagate by recruitment of cellular prion protein (1). The function of the cellular prion protein (PrP) is still poorly understood. It has been proposed that one unprecedented role for PrP is against Bax-mediated neuronal apoptosis. It has been shown that PrP potently inhibits Bax-induced cell death in human primary neurons (2). An impaired synaptic inhibition may be involved in the epileptiform activity seen in Creutzfeldt-Jakob and other neurodegenerative diseases and it is believed that loss of function of PrP may contribute to the early synaptic loss and neuronal degeneration seen in these diseases (3).

Alternate Names

CD230, CJD, fatal familial insomnia), GSS, prion protein, prion protein (p27-30), prion protein PrP, prion-related protein, PRIPMGC26679

Gene Symbol

PRNP

Additional PrPC Products

Product Documents for PrPC Antibody (3F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PrPC Antibody (3F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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