PRPF31 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55409

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-55409
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PRPF31 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KELGNSLDKCKNNEN corresponding to internal region according to NP_056444.3.

Epitope

KELGNSLDKCKNNEN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRPF31 Antibody

Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: PRPF31 Antibody [NB100-55409]

Flow Cytometry: PRPF31 Antibody [NB100-55409]

Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
View 2 more images

Applications for PRPF31 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in A431, HeLa, HEK293 and NIH3T3 lysates (calculated MW of 55.4 kDa band according to NP_056444.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRPF31

Retinitis pigmentosa (RP) is a clinically and genetically heterogeneous group of retinal dystrophies characterized by a progressive degeneration of photoreceptors, eventually resulting in severe visual impairment.

Alternate Names

DKFZp566J153, hPrp31, NY-BR-99, Pre-mRNA-processing factor 31, Protein 61K, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), PRP31 pre-mRNA processing factor 31 homolog (yeast), PRP31RP11, Serologically defined breast cancer antigen NY-BR-99, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6 snRNP 61 kDa protein

Entrez Gene IDs

26121 (Human); 68988 (Mouse)

Gene Symbol

PRPF31

UniProt

NP_056444.2 (Human)

Additional PRPF31 Products

Product Documents for PRPF31 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRPF31 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.