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PRPF4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82999

Novus Biologicals, part of Bio-Techne
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NBP1-82999
NBP1-82999-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF4B Antibody

Western Blot: PRPF4B Antibody [NBP1-82999]

Western Blot: PRPF4B Antibody [NBP1-82999]

Western Blot: PRPF4B Antibody [NBP1-82999] - Analysis in human cell line HELA.
Immunocytochemistry/ Immunofluorescence: PRPF4B Antibody [NBP1-82999]

Immunocytochemistry/ Immunofluorescence: PRPF4B Antibody [NBP1-82999]

Immunocytochemistry/Immunofluorescence: PRPF4B Antibody [NBP1-82999] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999]

Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999]

Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999] - Staining of human urinary bladder shows strong nuclear and cytoplasmic positivity in urothelial cells.

Applications for PRPF4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRPF4B

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq]

Alternate Names

dJ1013A10.1, dJ1013A10.1 (PRP4 protein kinase homolog), KIAA0536EC 2.7.11.1, PR4H, protein-serine/threonine kinase, PRP4 kinase, PRP4 pre-mRNA processing factor 4 homolog B (yeast), PRP4 pre-mRNA-processing factor 4 homolog, PRP4H, PRP4K, PRP4Prp4, serine/threonine-protein kinase PRP4 homolog

Gene Symbol

PRPF4B

Additional PRPF4B Products

Product Documents for PRPF4B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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