PRRX1 Antibody (OTI1E10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73678

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PRRX1 Antibodies »

Product Summary for PRRX1 Antibody (OTI1E10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human PRRX1 (NP_073207) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PRRX1 Antibody (OTI1E10) - Azide and BSA Free

Western Blot: PRRX1 Antibody (OTI1E10)Azide and BSA Free [NBP2-73678]

Western Blot: PRRX1 Antibody (OTI1E10)Azide and BSA Free [NBP2-73678]

Western Blot: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRRX1.
Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) - Azide and BSA Free [NBP2-73678] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for PRRX1 Antibody (OTI1E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRRX1

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns.

Alternate Names

Homeobox protein PHOX1, paired mesoderm homeo box 1, paired mesoderm homeobox 1 isoform pmx-1b, paired mesoderm homeobox protein 1, paired related homeobox 1, Paired-related homeobox protein 1, PHOX1PRX-1, PMX1PRX1

Gene Symbol

PRRX1

Additional PRRX1 Products

Product Documents for PRRX1 Antibody (OTI1E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRRX1 Antibody (OTI1E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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