Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E3

Concentration

1 mg/ml

View all PSD-95 Antibodies »

Product Specifications

Immunogen

Recombinant rat PSD-95

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Axon

Specificity

Detects ~100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.

Marker

post-Synaptic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PSD-95 Antibody (7E3)

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.
Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Immunohistochemistry analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice. .
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Applications for PSD-95 Antibody (7E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/mL was sufficient for detection of PSD-95 on 20 ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Use in IHC-Fr reported by customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-12872 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSD-95

1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse igG: HRP as the secondary.

Long Name

Postsynaptic Density Protein 95/Disks Large Homolog 4

Alternate Names

DLG4, PSD95, SAP90

Gene Symbol

DLG4

UniProt

P31016 (Rat)

Additional PSD-95 Products

Product Documents for PSD-95 Antibody (7E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSD-95 Antibody (7E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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