Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PSGL1/1601

Format

Azide and BSA Free

Concentration

1 mg/ml

View all PSGL-1/CD162 Antibodies »

Product Summary for PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free

Immunogen

Recombinant human PSGL-1/CD162 protein (Uniprot: Q14242)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53344).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380] - Formalin-fixed, paraffin-embedded human Spleen stained withCD162 Monoclonal Antibody (PSGL1/1601).
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free [NBP2-54380] - Formalin-fixed, paraffin-embedded human Tonsil stained withCD162 Monoclonal Antibody (PSGL1/1601).

Applications for PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSGL-1/CD162 Antibody (PSGL1/1601) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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