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PSGL-1/CD162 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16974

Novus Biologicals, part of Bio-Techne
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NBP3-16974-100ul
NBP3-16974-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PSGL-1/CD162 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSRKGHMYPVRNYSPTEMVCISSLLPDGGEGPSATANGGLSKAKSPGLTPEPREDREGDDLTLHSFLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSGL-1/CD162 Antibody

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974] - Analysis in human lymph node and skeletal muscle tissues using Anti-SELPLG antibody. Corresponding SELPLG RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody [NBP3-16974] - Staining of human skeletal muscle shows low expression as expected.

Applications for PSGL-1/CD162 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSGL-1/CD162 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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