Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Rat, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GCP-04

Format

BSA Free

Concentration

1.0 mg/ml

View all PSMA/FOLH1/NAALADase I Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells (NM_001014986.1).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Canine reactivity reported from a verified customer review.

Specificity

The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04)BSA Free [NBP1-45057]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04)BSA Free [NBP1-45057]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Analysis of human PSMA using mouse monoclonal antibody GCP-04 on lysates of LNCaP cell line and Jurkat cell line (PSMA non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-PSMA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free [NBP1-45057]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free [NBP1-45057]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.
Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04)BSA Free [NBP1-45057]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04)BSA Free [NBP1-45057]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.
View 4 more images

Applications for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Western Blot

1 ug/ml
Application Notes
PSMA/FOLH1/NAALADase I antibody validated for WB from a verified customer review. Western blotting: Recommended dilution: 1 ug/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-45057 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSMA/FOLH1/NAALADase I

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human); 85309 (Rat); 397677 (Rat)

Gene Symbol

FOLH1

UniProt

Q04609

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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