PSMA1 Antibody (3F12-2C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005682-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F12-2C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSMA1 Antibodies »

Product Specifications

Immunogen

PSMA1 (AAH02577, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH

Specificity

PSMA1 - proteasome (prosome, macropain) subunit, alpha type, 1 (3F12-2C9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMA1 Antibody (3F12-2C9)

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (3F12-2C9) [H00005682-M02]

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (3F12-2C9) [H00005682-M02]

Immunocytochemistry/Immunofluorescence: PSMA1 Antibody (3F12-2C9) [H00005682-M02] - Analysis of monoclonal antibody to PSMA1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PSMA1 Antibody (3F12-2C9) [H00005682-M02] - Detection limit for recombinant GST tagged PSMA1 is approximately 3ng/ml as a capture antibody.

Applications for PSMA1 Antibody (3F12-2C9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMA1

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Proteasome (Prosome, Macropain) Subunit, alpha Type, 1

Alternate Names

Macropain Subunit C2, P30-33K, PROS30, Proteasome Component C2, Proteasome nu Chain, PSC2

Entrez Gene IDs

5682 (Human)

Gene Symbol

PSMA1

UniProt

AAH02577 (Human)

Additional PSMA1 Products

Product Documents for PSMA1 Antibody (3F12-2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMA1 Antibody (3F12-2C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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