PSMA4 Antibody (2A10-E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005685-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2A10-E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSMA4 Antibodies »

Product Specifications

Immunogen

PSMA4 (AAH05361, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK

Specificity

PSMA4 - proteasome (prosome, macropain) subunit, alpha type, 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMA4 Antibody (2A10-E4)

Western Blot: PSMA4 Antibody (2A10-E4) [H00005685-M01]

Western Blot: PSMA4 Antibody (2A10-E4) [H00005685-M01]

Western Blot: PSMA4 Antibody (2A10-E4) [H00005685-M01] - PSMA4 monoclonal antibody (M01), clone 2A10-E4 Analysis of PSMA4 expression in Hela.
Immunocytochemistry/ Immunofluorescence: PSMA4 Antibody (2A10-E4) [H00005685-M01]

Immunocytochemistry/ Immunofluorescence: PSMA4 Antibody (2A10-E4) [H00005685-M01]

Immunocytochemistry/Immunofluorescence: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Analysis of monoclonal antibody to PSMA4 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Detection limit for recombinant GST tagged PSMA4 is approximately 3ng/ml as a capture antibody.

Applications for PSMA4 Antibody (2A10-E4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00005685-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMA4

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Proteasome subunit alpha type-4

Alternate Names

HC9, PSC9

Entrez Gene IDs

5685 (Human)

Gene Symbol

PSMA4

UniProt

AAH05361 (Human)

Additional PSMA4 Products

Product Documents for PSMA4 Antibody (2A10-E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMA4 Antibody (2A10-E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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