PSMA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52074

Novus Biologicals, part of Bio-Techne
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NBP1-52074
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PSMA4 Antibodies »

Product Summary for PSMA4 Antibody

Immunogen

Peptide with sequence C-SDANVLTNELR corresponding to internal region according to NP_001096138.1.

Epitope

C-SDANVLTNELR

Specificity

This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMA4 Antibody

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PSMA4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in K562, NIH3T3 lysates and human tonsil, human peripheral blood mononucleocytes and in mouse and rat spleen lysates (calculated MW of 29.5 kDa band according to NP_002780.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMA4

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Proteasome subunit alpha type-4

Alternate Names

HC9, PSC9

Entrez Gene IDs

5685 (Human); 26441 (Mouse); 29671 (Rat)

Gene Symbol

PSMA4

UniProt

NP_002780.1 (Human)

Additional PSMA4 Products

Product Documents for PSMA4 Antibody

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Certificate of Analysis

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Product Specific Notices for PSMA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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