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PSMA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52074

Novus Biologicals, part of Bio-Techne
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NBP1-52074

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SDANVLTNELR corresponding to internal region according to NP_001096138.1.

Epitope

C-SDANVLTNELR

Specificity

This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMA4 Antibody

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074]

Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PSMA4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in K562, NIH3T3 lysates and human tonsil, human peripheral blood mononucleocytes and in mouse and rat spleen lysates (calculated MW of 29.5 kDa band according to NP_002780.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMA4

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Proteasome subunit alpha type-4

Alternate Names

HC9, PSC9

Entrez Gene IDs

5685 (Human); 26441 (Mouse); 29671 (Rat)

Gene Symbol

PSMA4

Additional PSMA4 Products

Product Documents for PSMA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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