PSMB10/MECL1 Antibody (PSH01-58)

Western Blot: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863]

Western Blot: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Western blot analysis of PSMB10/MECL1 on different lysates with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution.

Lane 1: HeLa treated with 100ng/mL IFN- for 48 hours cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: Daudi cell lysate (20 ug/Lane)
Lane 4: Raji cell lysate (20 ug/Lane)
Lane 5: THP-1 cell lysate (20 ug/Lane)
Lane 6: Ramos cell lysate (20 ug/Lane)
Lane 7: U-937 cell lysate (20 ug/Lane)
Lane 8: HL-60 cell lysate (20 ug/Lane)
Lane 9: Jurkat cell lysate (20 ug/Lane)
Lane 10: SiHa cell lysate (20 ug/Lane)
Lane 11: Mouse spleen tissue lysate (40 ug/Lane)
Lane 12: Mouse thymus tissue lysate (40 ug/Lane)

Predicted band size: 29 kDa
Observed band size: 25/29 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32863) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863]

Immunohistochemistry: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.