PSMC6 Antibody (2C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005706-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSMC6 Antibodies »

Product Specifications

Immunogen

PSMC6 (AAH05390, 290 a.a. ~ 389 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PSMC6 (2C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMC6 Antibody (2C4)

Western Blot: PSMC6 Antibody (2C4) [H00005706-M02]

Western Blot: PSMC6 Antibody (2C4) [H00005706-M02]

Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 expression in transfected 293T cell line by PSMC6 monoclonal antibody (M02), clone 2C4.Lane 1: PSMC6 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02]

Immunocytochemistry/ Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02]

Immunocytochemistry/Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of monoclonal antibody to PSMC6 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PSMC6 Antibody (2C4) [H00005706-M02]

Western Blot: PSMC6 Antibody (2C4) [H00005706-M02]

Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 over-expressed 293 cell line, cotransfected with PSMC6 Validated Chimera RNAi ( Cat # H00005706-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMC6 monoclonal antibody (M02), clone 2C4 (Cat # H00005706-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for PSMC6 Antibody (2C4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMC6

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12.

Alternate Names

26S protease regulatory subunit 10B, 26S protease regulatory subunit S10B, 26S proteasome AAA-ATPase subunit RPT4, CADP44, conserved ATPase domain protein 44, MGC12520, p42, P44, proteasome (prosome, macropain) 26S subunit, ATPase, 6, Proteasome 26S subunit ATPase 6, Proteasome subunit p42, SUG2

Entrez Gene IDs

5706 (Human)

Gene Symbol

PSMC6

OMIM

602708 (Human)

UniProt

AAH05390 (Human)

Additional PSMC6 Products

Product Documents for PSMC6 Antibody (2C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMC6 Antibody (2C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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