Key Product Details

Species Reactivity

Validated:

Human, Mouse, C. elegans

Cited:

Human, Nematode - Caenorhabditis elegans

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F4

Format

BSA Free

Concentration

1.0 mg/ml

View all PSMD11 Antibodies »

Product Specifications

Immunogen

Recombinant human PSMD11 (1-422aa) purified from E. coli (NP_002806).

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 22922647). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

47.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMD11 Antibody (1F4) - BSA Free

Western Blot: PSMD11 Antibody (1F4)BSA Free [NBP1-30252]

Western Blot: PSMD11 Antibody (1F4)BSA Free [NBP1-30252]

Western Blot: PSMD11 Antibody (1F4) [NBP1-30252] - The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for PSMD11 Antibody (1F4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:250-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD11

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Alternate Names

MGC3844,26S proteasome regulatory subunit RPN6, p44.5,26S proteasome regulatory subunit 9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5, Rpn6,26S proteasome regulatory subunit S9, S9,26S proteasome non-ATPase regulatory subunit 11

Entrez Gene IDs

5717 (Human)

Gene Symbol

PSMD11

UniProt

O00231 (Human)

Additional PSMD11 Products

Product Documents for PSMD11 Antibody (1F4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSMD11 Antibody (1F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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