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PSMD11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46192

Novus Biologicals, part of Bio-Techne
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NBP1-46192

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 372 and 422 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).

Reactivity Notes

X.tropicalis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD11 Antibody

Western Blot: PSMD11 Antibody [NBP1-46192]

Western Blot: PSMD11 Antibody [NBP1-46192]

Western Blot: PSMD11 Antibody [NBP1-46192] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PSMD11 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PSMD11 was also immunoprecipitated by rabbit anti-PSMD11 antibody NBP1-46191, which recognizes an upstream epitope.

Applications for PSMD11 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSMD11

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Alternate Names

MGC3844,26S proteasome regulatory subunit RPN6, p44.5,26S proteasome regulatory subunit 9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5, Rpn6,26S proteasome regulatory subunit S9, S9,26S proteasome non-ATPase regulatory subunit 11

Gene Symbol

PSMD11

Additional PSMD11 Products

Product Documents for PSMD11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMD11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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