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PSMD14 Antibody (5V0H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16837

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16837-100ul
NBP3-16837-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5V0H3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 112-210 of human PSMD14 (O00487). YHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD14 Antibody (5V0H3)

Western Blot: PSMD14 Antibody (5V0H3) [NBP3-16837]

Western Blot: PSMD14 Antibody (5V0H3) [NBP3-16837]

Western Blot: PSMD14 Antibody (5V0H3) [NBP3-16837] - Western blot analysis of extracts of various cell lines, using PSMD14 Rabbit mAb (NBP3-16837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PSMD14 Antibody (5V0H3) [NBP3-16837]

Immunocytochemistry/ Immunofluorescence: PSMD14 Antibody (5V0H3) [NBP3-16837]

Immunocytochemistry/Immunofluorescence: PSMD14 Antibody (5V0H3) [NBP3-16837] - Immunofluorescence analysis of U-2 OS cells using PSMD14 Rabbit mAb (NBP3-16837) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PSMD14 Antibody (5V0H3) [NBP3-16837]

Immunohistochemistry-Paraffin: PSMD14 Antibody (5V0H3) [NBP3-16837]

Immunohistochemistry-Paraffin: PSMD14 Antibody (5V0H3) [NBP3-16837] - Immunohistochemistry of paraffin-embedded mouse heart using PSMD14 Rabbit mAb (NBP3-16837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for PSMD14 Antibody (5V0H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMD14

PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).[supplied by OMIM]

Alternate Names

EC 3.1.2.15, EC 3.4.19.-, pad1, POH126S proteasome non-ATPase regulatory subunit 14,26S proteasome regulatory subunit RPN11, proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, Rpn11, RPN11,26S proteasome-associated PAD1 homolog 1

Gene Symbol

PSMD14

Additional PSMD14 Products

Product Documents for PSMD14 Antibody (5V0H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMD14 Antibody (5V0H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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