PSMF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98614

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PSMF1 Antibodies »

Product Summary for PSMF1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human PSMF1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PSMF1 Antibody

Western Blot: PSMF1 Antibody [NBP2-98614]

Western Blot: PSMF1 Antibody [NBP2-98614]

Western Blot: PSMF1 Antibody [NBP2-98614] - Anti-PSMF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa
Immunohistochemistry-Paraffin: PSMF1 Antibody [NBP2-98614]

Immunohistochemistry-Paraffin: PSMF1 Antibody [NBP2-98614]

Immunohistochemistry-Paraffin: PSMF1 Antibody [NBP2-98614] - Immunochemical staining of human PSMF1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for PSMF1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMF1

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Alternate Names

hPI31, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), proteasome inhibitor hP131 subunit, proteasome inhibitor PI31 subunit

Gene Symbol

PSMF1

Additional PSMF1 Products

Product Documents for PSMF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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