PSPC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83801

Novus Biologicals, part of Bio-Techne
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NBP1-83801
NBP1-83801-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSPC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PAMGPEGAANMGTPMMPDNGAVHNDRFPQGPPSQMGSPMGSRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSPC1 Antibody

Western Blot: PSPC1 Antibody [NBP1-83801]

Western Blot: PSPC1 Antibody [NBP1-83801]

Western Blot: PSPC1 Antibody [NBP1-83801] - Analysis in human cell lines Caco-2 and HeLa using Anti-PSPC1 antibody. Corresponding PSPC1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PSPC1 Antibody [NBP1-83801]

Immunocytochemistry/ Immunofluorescence: PSPC1 Antibody [NBP1-83801]

Immunocytochemistry/Immunofluorescence: PSPC1 Antibody [NBP1-83801] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining shown in green.
Immunohistochemistry-Frozen: PSPC1 Antibody [NBP1-83801]

Immunohistochemistry-Frozen: PSPC1 Antibody [NBP1-83801]

Immunohistochemistry-Frozen: PSPC1 Antibody [NBP1-83801] - Rat brain tissue. Fresh frozen 16 um. PSPC1 (1:500, green), DAPI (blue). IHC-Fr image submitted by a verified customer review.
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Applications for PSPC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Frozen

Validated from a verified customer review

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-83801 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSPC1

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line . Binds to poly(A), poly(G) and poly(U) RNA homopolymers

Alternate Names

FLJ33554, paraspeckle component 1, paraspeckle protein 1, PSP1

Gene Symbol

PSPC1

Additional PSPC1 Products

Product Documents for PSPC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSPC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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