PSPC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12643

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PSPC1 Antibodies »

Product Summary for PSPC1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human PSPC1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for PSPC1 Antibody

Western Blot: PSPC1 Antibody [NBP3-12643]

Western Blot: PSPC1 Antibody [NBP3-12643]

Western Blot: PSPC1 Antibody [NBP3-12643] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: PSPC1 Antibody [NBP3-12643]

Immunocytochemistry/ Immunofluorescence: PSPC1 Antibody [NBP3-12643]

Immunocytochemistry/Immunofluorescence: PSPC1 Antibody [NBP3-12643] - Staining of PSPC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PSPC1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: PSPC1 Antibody [NBP3-12643]

Immunohistochemistry-Paraffin: PSPC1 Antibody [NBP3-12643]

Immunohistochemistry-Paraffin: PSPC1 Antibody [NBP3-12643] - Staining of human PSPC1 in human testis with rabbit polyclonal antibody at 1:2000 dilution.
View 1 more images

Applications for PSPC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:500-1:3000

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSPC1

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line . Binds to poly(A), poly(G) and poly(U) RNA homopolymers

Alternate Names

FLJ33554, paraspeckle component 1, paraspeckle protein 1, PSP1

Gene Symbol

PSPC1

Additional PSPC1 Products

Product Documents for PSPC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSPC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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