PTEN Antibody (CL11723)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21201

Novus Biologicals, part of Bio-Techne
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NBP3-21201-100ul
NBP3-21201-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL11723

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PTEN Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Synthetic Peptide of P60484. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PTEN Antibody (CL11723)

Western Blot: PTEN Antibody [NBP3-21201] -

Western Blot: PTEN Antibody [NBP3-21201] -

Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
View 4 more images

Applications for PTEN Antibody (CL11723)

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTEN

PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosporylation of the tail of the protein will suppress its activity and modify its conformation to a

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Gene Symbol

PTEN

Additional PTEN Products

Product Documents for PTEN Antibody (CL11723)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PTEN Antibody (CL11723)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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