PTEN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2229

Novus Biologicals, part of Bio-Techne
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NB100-2229
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PTEN Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 403) of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTEN Antibody

Western Blot: PTEN Antibody [NB100-2229]

Western Blot: PTEN Antibody [NB100-2229]

Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.
Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229]

Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229]

Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PTEN used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PTEN Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PTEN

The PTEN tumor suppressor gene acts as a phospholipid phosphatase. Under normal growth conditions, stimulatory signals from the insulin receptor activate the enzyme PI3-K, which phosphorylates phosphotidylinositol 4,5-bisphosphate (PIP2) to generate phosphotidylinositol 3,4,5-triphosphate (PIP3), a lipid molecule. Downstream, PIP3 activates several effectors, including the proto-oncogene product PKB/Akt. The role of PTEN is to dephosphorylate PIP3, acting as a negative control on PKB/Akt activation. If a mutation in PTEN causes it unable to carry out its phosphatase function, PIP3 can no longer be deactivated, so it continues to propagate its signal downstream. This may result in the continued activation of PKB/Akt, which, in combination with other factors, could lead to increased cell growth and possible tumor development

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Entrez Gene IDs

5728 (Human); 19211 (Mouse)

Gene Symbol

PTEN

UniProt

P60484 (Human)

Additional PTEN Products

Product Documents for PTEN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTEN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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