PTENP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-86973
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified PTENP1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTENP1 protein. (Uniprot: O43460)
Reactivity Notes
A BLAST analysis was used to suggest cross reactivity with PTENP1 proteins from mouse, dog sources based on 82% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against human PTENP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PTENP1 Antibody
![Western Blot: PTENP1 Antibody [NB110-86973]](https://resources.bio-techne.com/images/products/PTENP1-Antibody-Western-Blot-NB110-86973-img0003.jpg "Western Blot: PTENP1 Antibody [NB110-86973]")
Western Blot: PTENP1 Antibody [NB110-86973]
Western Blot: PTENP1 Antibody [NB110-86973] - Analysis of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6). The band at 55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).Applications for PTENP1 Antibody
Application
Recommended Usage
ELISA
1:160000
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PTENP1
Alternate Names
phosphatase and tensin homolog pseudogene 1, psiPTEN, PTEN2, PTH2, PTEN-rs
Gene Symbol
PTENP1
UniProt
Additional PTENP1 Products
Product Documents for PTENP1 Antibody
Product Specific Notices for PTENP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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