PTH1R/PTHR1 Antibody (JE36-10)

Western Blot: PTH1R/PTHR1 Antibody (JE36-10) [NBP3-32876]

Western Blot: PTH1R/PTHR1 Antibody (JE36-10) [NBP3-32876] - Western blot analysis of PTH1R/PTHR1 on different lysates with Rabbit anti-PTH1R/PTHR1 antibody (NBP3-32876) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: THP-1 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32876) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.