Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F2

Concentration

0.58 mg/ml

View all PTP epsilon Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTP epsilon Antibody (OTI5F2)

Western Blot: PTP epsilon Antibody (OTI5F2) [NBP2-02374]

Western Blot: PTP epsilon Antibody (OTI5F2) [NBP2-02374]

Western Blot: PTP epsilon Antibody (5F2) [NBP2-02374] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilon.
Immunocytochemistry/Immunofluorescence: PTP epsilon Antibody (5F2) [NBP2-02374] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP epsilon.
Immunohistochemistry-Paraffin: PTP epsilon Antibody (OTI5F2) [NBP2-02374]

Immunohistochemistry-Paraffin: PTP epsilon Antibody (OTI5F2) [NBP2-02374]

Immunohistochemistry-Paraffin: PTP epsilon Antibody (5F2) [NBP2-02374] - Staining of paraffin-embedded prostate tissue using anti-PTP epsilon mouse monoclonal antibody.
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Applications for PTP epsilon Antibody (OTI5F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.58 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTP epsilon

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported,one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region,and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels.

Alternate Names

DKFZp313F1310, EC 3.1.3.48, FLJ57799, FLJ58245, HPTPE, protein tyrosine phosphatase epsilon, protein tyrosine phosphatase, receptor type, E, protein tyrosine phosphatase, receptor type, epsilon polypeptide, Protein-tyrosine phosphatase epsilon, PTPE, receptor-type tyrosine-protein phosphatase epsilon, R-PTP-epsilon

Entrez Gene IDs

5791 (Human)

Gene Symbol

PTPRE

Additional PTP epsilon Products

Product Documents for PTP epsilon Antibody (OTI5F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PTP epsilon Antibody (OTI5F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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