PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52543

Novus Biologicals, part of Bio-Techne
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NBP2-52543-0.025mg
NBP2-52543
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F8F11

Format

BSA Free

Concentration

1 mg/ml

View all PTP1B Antibodies »

Product Summary for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Immunogen

Purified recombinant fragment of human PTP1B/PTPN1 (AA: 40-246) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Western Blot: PTP1B/PTPN1 Antibody (4F8F11)BSA Free [NBP2-52543]

Western Blot: PTP1B/PTPN1 Antibody (4F8F11)BSA Free [NBP2-52543]

Western Blot: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis using PTPN1 mouse mAb against Jurkat (1), MCF-7 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (4F8F11) - BSA Free [NBP2-52543]

Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (4F8F11) - BSA Free [NBP2-52543]

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis of Hela cells using PTPN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (4F8F11) - BSA Free [NBP2-52543]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (4F8F11) - BSA Free [NBP2-52543]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (4F8F11) [NBP2-52543] - Analysis of paraffin-embedded bladder cancer tissues using PTPN1 mouse mAb with DAB staining.
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Applications for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTP1B/PTPN1 Antibody (4F8F11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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