PTP1B/PTPN1 [p Ser50] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23195

Novus Biologicals, part of Bio-Techne
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NBP3-23195
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PTP1B Antibodies »

Product Summary for PTP1B/PTPN1 [p Ser50] Antibody

Immunogen

PTP1B/PTPN1 (Ser-50) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-50 in human PTP1B/PTPN1. This sequence has significant homology to the conserved site in rat and mouse PTP1B/PTPN1, and has homology to conserved sites in TC-PTP and MPTP, but not other protein tyrosine phosphatase family members. [UniProt# P18031]

Modification

p Ser50

Specificity

The antibody detects a 50 kDa* doublet corresponding to PTP1B/PTPN1 in western blots of calyculin A treated human A431 and Jurkat cells. This reactivity is observed as a weak singlet in control cells and is not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PTP1B/PTPN1 [p Ser50] Antibody

PTP1B/PTPN1 [p Ser50] Antibody

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 [p Ser50] Antibody [NBP3-23195] -

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 [p Ser50] Antibody [NBP3-23195] - Immunocytochemical labeling of PTP1B/PTPN1 in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-PTP1B/PTPN1 (Ser-50) (NBP3-23195) phosphospecific antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
PTP1B/PTPN1 [p Ser50] Antibody

Western Blot: PTP1B/PTPN1 [p Ser50] Antibody [NBP3-23195] -

Western Blot: PTP1B/PTPN1 [p Ser50] Antibody [NBP3-23195] - Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B/PTPN1 (lanes 1 & 2) or rabbit polyclonal anti-PTP1B/PTPN1 (Ser-50) (lanes 3 & 4).

Applications for PTP1B/PTPN1 [p Ser50] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 [p Ser50] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTP1B/PTPN1 [p Ser50] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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