PTPN12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60666

Novus Biologicals, part of Bio-Techne
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NB100-60666
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PTPN12 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 760 and 780 of human protein tyrosine phosphatase, non-receptor type 12 using the numbering given in entry NP_002826.2 (GeneID 5782).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTPN12 Antibody

Western Blot: PTPN12 Antibody [NB100-60666]

Western Blot: PTPN12 Antibody [NB100-60666]

Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody NB100-60666 used at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunohistochemistry: PTPN12 Antibody [NB100-60666]

Immunohistochemistry: PTPN12 Antibody [NB100-60666]

Immunohistochemistry: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human stomach carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- PTPN12 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: PTPN12 Antibody [NB100-60666]

Western Blot: PTPN12 Antibody [NB100-60666]

Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12/PTP-PEST on HeLa whole cell lysate using NB100-60666. PTPN12/PTPPEST was also IPed by NB100-60663, NB100-60664, and NB100-60665.
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Applications for PTPN12 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PTPN12

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Long Name

Tyrosine-protein phosphatase non-receptor type 12

Alternate Names

PTP-PEST, PTPG1

Entrez Gene IDs

5782 (Human); 19248 (Mouse)

Gene Symbol

PTPN12

UniProt

Q05209 (Human)

Additional PTPN12 Products

Product Documents for PTPN12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTPN12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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