PTPN12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87188

Novus Biologicals, part of Bio-Techne
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NBP1-87188
NBP1-87188-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PTPN12 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVTQSNKVSVTPPEESQNSDTPPRPDRLPLDEKGHVTWSFHGPENAIPIPDLSEGNSSDINYQTRKTVSLTPSPTTQVETPDLVDHDNTSPLFRTPLSFTNPLHSDDSDSDERNSDGAVTQNKTNISTASATVSAAT

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435421)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTPN12 Antibody

Western Blot: PTPN12 Antibody [NBP1-87188]

Western Blot: PTPN12 Antibody [NBP1-87188]

Western Blot: PTPN12 Antibody [NBP1-87188] - Analysis in human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody [NBP1-87188]

Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody [NBP1-87188]

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody [NBP1-87188] - Staining of human cell line U-251 MG shows positivity in plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP1-87188]

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP1-87188]

Immunohistochemistry-Paraffin: PTPN12 Antibody [NBP1-87188] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Applications for PTPN12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 25868976). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTPN12

PTPN12 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Long Name

Tyrosine-protein phosphatase non-receptor type 12

Alternate Names

PTP-PEST, PTPG1

Gene Symbol

PTPN12

Additional PTPN12 Products

Product Documents for PTPN12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTPN12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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