PTPN3 [p Ser459] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29518

Novus Biologicals, part of Bio-Techne
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NBP2-29518
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Finch (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PTPN3 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 conjugated to KLH. Accession # P26045

Modification

p Ser459

Specificity

Specific for endogenous levels of the ~104 kDa PTPN3 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPN3 [p Ser459] Antibody

Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518]

Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518]

Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for PTPN3 [p Ser459] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPN3

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38gamma MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38 gamma and PTPH1 protein expression, and there is a coupling of increased p38gamma and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).

Long Name

Tyrosine-protein phosphatase non-receptor type 3

Alternate Names

PTP-H1, PTPH1

Entrez Gene IDs

5774 (Human)

Gene Symbol

PTPN3

UniProt

P26045 (Human)

Additional PTPN3 Products

Product Documents for PTPN3 [p Ser459] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTPN3 [p Ser459] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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