Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PTPN7 Antibodies »

Product Summary for PTPN7 Antibody (OTI3B10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human PTPN7(NP_542155) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPN7 Antibody (OTI3B10) - Azide and BSA Free

Western Blot: PTPN7 Antibody (OTI3B10)Azide and BSA Free [NBP2-73732]

Western Blot: PTPN7 Antibody (OTI3B10)Azide and BSA Free [NBP2-73732]

Western Blot: PTPN7 Antibody (OTI3B10) - Azide and BSA Free [NBP2-73732] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN7.
Immunocytochemistry/ Immunofluorescence: PTPN7 Antibody (OTI3B10) - Azide and BSA Free [NBP2-73732]

Immunocytochemistry/ Immunofluorescence: PTPN7 Antibody (OTI3B10) - Azide and BSA Free [NBP2-73732]

Immunocytochemistry/Immunofluorescence: PTPN7 Antibody (OTI3B10) - Azide and BSA Free [NBP2-73732] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN7.
Western Blot: PTPN7 Antibody (OTI3B10)Azide and BSA Free [NBP2-73732]

Western Blot: PTPN7 Antibody (OTI3B10)Azide and BSA Free [NBP2-73732]

Western Blot: PTPN7 Antibody (OTI3B10) - Azide and BSA Free [NBP2-73732] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN7 monoclonal antibody.

Applications for PTPN7 Antibody (OTI3B10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPN7

PTPN7 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Tyrosine-protein phosphatase non-receptor type 7

Alternate Names

BPTP-4, FLJ18703, HEPTPnonreceptor-type, stress induced, LPTP, protein tyrosine phosphatase, non-receptor type 7, tyrosine-protein phosphatase non-receptor type 7

Gene Symbol

PTPN7

Additional PTPN7 Products

Product Documents for PTPN7 Antibody (OTI3B10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTPN7 Antibody (OTI3B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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