PTPRN2 Antibody (7F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005799-M08

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PTPRN2 Antibodies »

Product Specifications

Immunogen

PTPRN2 (NP_002838, 108 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ELADLPKTYLRRPEASSPARPSKHSVGSERRYSREGGAALANALRRHLPFLEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGPRTQL

Specificity

PTPRN2 (7F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PTPRN2 Antibody (7F8)

Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08]

Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08]

Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08] - Analysis of PTPRN2 expression in transfected 293T cell line by PTPRN2 monoclonal antibody (M08), clone 7F8. Lane 1: PTPRN2 transfected lysatE (108.1 KDa). Lane 2: Non-transfected lysate.
Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08]

Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08]

Western Blot: PTPRN2 Antibody (7F8) [H00005799-M08] - Western blot analysis of PTPRN2 over-expressed 293 cell line, cotransfected with PTPRN2 Validated Chimera RNAi or non-transfected control. Blot probed with H00005799-M08. GAPDH (36.1 kDa) used as loading control.
Sandwich ELISA: PTPRN2 Antibody (7F8) [H00005799-M08] - Detection limit for recombinant GST tagged PTPRN2 is 0.3 ng/ml as a capture antibody.

Applications for PTPRN2 Antibody (7F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTPRN2

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

Alternate Names

EC 3.1.3.48, IA-2beta, IAR/receptor-like protein-tyrosine phosphatase, ICAARIARPTPRP, Islet cell autoantigen-related protein, KIAA0387, phogrin, protein tyrosine phosphatase receptor pi, protein tyrosine phosphatase, receptor type, N polypeptide 2, receptor-type tyrosine-protein phosphatase N2, R-PTP-N2, tyrosine phosphatase IA-2 beta

Entrez Gene IDs

5799 (Human)

Gene Symbol

PTPRN2

OMIM

601698 (Human)

UniProt

NP_002838 (Human)

Additional PTPRN2 Products

Product Documents for PTPRN2 Antibody (7F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTPRN2 Antibody (7F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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