PTTG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20287

Novus Biologicals, part of Bio-Techne
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NBP2-20287
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PTTG1 Antibodies »

Product Summary for PTTG1 Antibody

Immunogen

Full length human PTTG1 Recombinant protein.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTTG1 Antibody

Western Blot: PTTG1 Antibody [NBP2-20287]

Western Blot: PTTG1 Antibody [NBP2-20287]

Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-20287]

Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-20287]

Immunocytochemistry/Immunofluorescence: PTTG1 Antibody [NBP2-20287] - Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-20287]

Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-20287]

Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-20287] - Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Securin antibody dilution: 1:500.
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Applications for PTTG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTTG1

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. [provided by RefSeq]

Alternate Names

EAP1MGC126883, Esp1-associated protein, ESP1-associated protein 1, hPTTG, pituitary tumor-transforming 1, Pituitary tumor-transforming gene 1 protein, PTTGMGC138276, securin, Tumor-transforming protein 1, TUTR1HPTTG

Gene Symbol

PTTG1

UniProt

O95997 (Human)

Additional PTTG1 Products

Product Documents for PTTG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTTG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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