PU.1/Spi-1 Antibody (PD00-14)

Western Blot: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878]

Western Blot: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878] - Western blot analysis of PU.1/Spi-1 on different lysates with Rabbit anti-PU.1/Spi-1 antibody (NBP3-32878) at 1/500 dilution.

Lane 1: Daudi cell lysate
Lane 2: THP-1 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 31 kDa
Observed band size: 40 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32878) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878]

Immunohistochemistry: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-PU.1/Spi-1 antibody (NBP3-32878) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32878) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.