PUF60 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-57501

Novus Biologicals, part of Bio-Techne
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NBP1-57501
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all PUF60 Antibodies »

Product Summary for PUF60 Antibody

Immunogen

Synthetic peptides corresponding to PUF60(poly-U binding splicing factor 60KDa) The peptide sequence was selected from the C terminal of PUF60. Peptide sequence EIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PUF60 Antibody

Western Blot: PUF60 Antibody [NBP1-57501]

Western Blot: PUF60 Antibody [NBP1-57501]

Western Blot: PUF60 Antibody [NBP1-57501] - Antibody Titration: 0.2-1 ug/ml Positive control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa.
Immunohistochemistry: PUF60 Antibody [NBP1-57501]

Immunohistochemistry: PUF60 Antibody [NBP1-57501]

Immunohistochemistry: PUF60 Antibody [NBP1-57501]
Western Blot: PUF60 Antibody [NBP1-57501]

Western Blot: PUF60 Antibody [NBP1-57501]

Western Blot: PUF60 Antibody [NBP1-57501] - Transfected 293T Cell Lysate 5ug/ml Gel Concentration 12%
View 1 more images

Applications for PUF60 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUF60

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

Alternate Names

FBP interacting repressor, FBP-interacting repressor, FIRpoly-U binding splicing factor PUF60, FLJ31379, FUSE-binding protein-interacting repressor, poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor 60KDa, Ro ribonucleoprotein binding protein 1, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, roBP1, ROBPI, siah binding protein 1,60 kDa poly(U)-binding-splicing factor, Siah-binding protein 1, Siah-BP1, SIAHBP1pyrimidine tract binding splicing factor

Gene Symbol

PUF60

UniProt

Q9UHX1-2 (Human)

Additional PUF60 Products

Product Documents for PUF60 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PUF60 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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