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PUM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20010

Novus Biologicals, part of Bio-Techne
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NBP2-20010

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PUM2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Chicken (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUM2 Antibody

Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-20010]

Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-20010]

Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-20010] - Paraffin-embedded mouse kidney PUM2 Antibody diluted at 1:500.
Western Blot: PUM2 Antibody [NBP2-20010]

Western Blot: PUM2 Antibody [NBP2-20010]

Western Blot: PUM2 Antibody [NBP2-20010] - Sample (20 ug of whole cell lysate) A: human ESC 5% SDS PAGE gel, diluted at 1:5000.
PUM2 Antibody

Western Blot: PUM2 Antibody [NBP2-20010] -

Western Blot: PUM2 Antibody [NBP2-20010] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PUM2 antibody [N1N2], N-term (NBP2-20010) diluted at 1:500.

Applications for PUM2 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PUM2

Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

Long Name

Pumilio 2

Alternate Names

PUMH2, Pumilio-2, PUML2

Gene Symbol

PUM2

UniProt

Additional PUM2 Products

Product Documents for PUM2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PUM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.