PUM3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87296

Novus Biologicals, part of Bio-Techne
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NBP1-87296
NBP1-87296-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PUM3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUM3 Antibody

Western Blot: PUM3 Antibody [NBP1-87296]

Western Blot: PUM3 Antibody [NBP1-87296]

Western Blot: KIAA0020 Antibody [NBP1-87296] - Analysis in control (vector only transfected HEK293T lysate) and KIAA0020 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [NBP1-87296]

Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [NBP1-87296]

Immunocytochemistry/Immunofluorescence: KIAA0020 Antibody [NBP1-87296] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]

Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]

Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human colon shows weak to moderate positivity in nucleoli in glandular cells.
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Applications for PUM3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUM3

Alternate Names

HBV X-transactivated gene 5 protein, HLA-HA8MGC8749, KIAA0020, KIAA0020 protein, Minor histocompatibility antigen HA-8, PEN, penguin homolog, protein 5 transactivated by hepatitis B virus X antigen (HBxAg), PUF6, XTP5HBV XAg-transactivated protein 5

Gene Symbol

KIAA0020

Additional PUM3 Products

Product Documents for PUM3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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