PUMA Antibody (SR1298)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21667

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21667-100ul
NBP3-21667-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1298

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PUMA Antibodies »

Product Summary for PUMA Antibody (SR1298)

Immunogen

A synthesized peptide derived from human PUMA (Uniprot #: Q9BXH1)

Specificity

Detects endogenous levels of total PUMA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUMA Antibody (SR1298)

Western Blot: PUMA Antibody (SR1298) [NBP3-21667] -

Western Blot: PUMA Antibody (SR1298) [NBP3-21667] - Western blot analysis using NBP3-21667 in K562 cell lysate.

Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR1298) [NBP3-21667] -

Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR1298) [NBP3-21667] - Immunofluorescent analysis of Hela cells, using NBP3-21667.

Immunohistochemistry: PUMA Antibody (SR1298) [NBP3-21667] -

Immunohistochemistry: PUMA Antibody (SR1298) [NBP3-21667] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21667.

Applications for PUMA Antibody (SR1298)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUMA

p53 upregulated modulator of apoptosis (PUMA), also called BBC3, is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Gene Symbol

BBC3

Additional PUMA Products

Product Documents for PUMA Antibody (SR1298)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PUMA Antibody (SR1298)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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