PUMA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56623

Novus Biologicals, part of Bio-Techne
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NB100-56623
NB100-56623SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all PUMA Antibodies »

Product Specifications

Immunogen

A synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36-53 of human PUMA d (GenBank no. gb|AAK39544.1|AF354656_1 ) and amino acids 196-213 of human PUMA g (GenBank no ref|NP_001120712.1|) was used as the immunogen.

Specificity

The amino acid sequence used as immunogen has 100% identity in PUMAd and PUMAg, and 59% identity in PUMAa. For additional information on PUMA isoforms and their sequences, see Nakano et al. 2001.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUMA Antibody

Western Blot: PUMA Antibody [NB100-56623]

Western Blot: PUMA Antibody [NB100-56623]

Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.

Applications for PUMA Antibody

Application
Recommended Usage

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUMA

A novel p53 inducible pro-apoptotic gene was identified and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Gene Symbol

BBC3

UniProt

NP_001120712.1 (Human)

Additional PUMA Products

Product Documents for PUMA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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