PUMA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52093

Novus Biologicals, part of Bio-Techne
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NBP1-52093
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PUMA Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DGGRPQRTVR corresponding to internal region according to NP_001120712.1.

Epitope

C-DGGRPQRTVR

Specificity

This antibody is expected to recognize isoform 1 (NP_001120712.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PUMA Antibody

Western Blot: PUMA Antibody [NBP1-52093]

Western Blot: PUMA Antibody [NBP1-52093]

Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PUMA Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 26.5 kDa band according to NP_001120712.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PUMA

PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Entrez Gene IDs

27113 (Human)

Gene Symbol

BBC3

UniProt

NP_001120712.1 (Human)

Additional PUMA Products

Product Documents for PUMA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PUMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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