Purine Nucleoside Phosphorylase/PNP Antibody (6E5)
Novus Biologicals, part of Bio-Techne | Catalog # H00004860-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 6E5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NP (NP_000261.1, 1 a.a. ~ 289 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS
Specificity
This product is specific for Human NP monoclonal antibody (M01), clone 6E5 [Gene ID: 4860].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Purine Nucleoside Phosphorylase/PNP Antibody (6E5)
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (6E5) [H00004860-M01]](https://resources.bio-techne.com/images/products/Purine-Nucleoside-Phosphorylase-PNP-Antibody-6E5-Western-Blot-H00004860-M01-img0002.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (6E5) [H00004860-M01]")
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (6E5) [H00004860-M01]
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (6E5) [H00004860-M01] - Analysis of NP expression in transfected 293T cell line by NP monoclonal antibody (M01), clone 6E5.Lane 1: NP transfected lysate (Predicted MW: 32.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Purine Nucleoside Phosphorylase/PNP Antibody (6E5) [H00004860-M01] - Detection limit for recombinant GST tagged NP is 10 ng/ml as a capture antibody.
Applications for Purine Nucleoside Phosphorylase/PNP Antibody (6E5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Purine Nucleoside Phosphorylase/PNP
Additional Purine Nucleoside Phosphorylase/PNP Products
Product Documents for Purine Nucleoside Phosphorylase/PNP Antibody (6E5)
Product Specific Notices for Purine Nucleoside Phosphorylase/PNP Antibody (6E5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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