Purine Nucleoside Phosphorylase/PNP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82541

Novus Biologicals, part of Bio-Techne
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NBP1-82541
NBP1-82541-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Purine Nucleoside Phosphorylase/PNP Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERGAPHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Purine Nucleoside Phosphorylase/PNP Antibody

Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining in human epididymis and testis tissues using anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same tissues.
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Analysis in human cell lines HEK293 and SK-MEL-30 using Anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Immunocytochemistry/ Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541]

Immunocytochemistry/Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining of human cell line RPTEC TERT1 shows localization to cytosol. Antibody staining is shown in green.
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Applications for Purine Nucleoside Phosphorylase/PNP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Purine Nucleoside Phosphorylase/PNP

The enzyme purine nucleoside phosphorylase, together with adenosine deaminase (ADA) serves a key role in purine catabolism. Mutations in either enzyme result in a severe combined immunodeficiency (SCID).

Alternate Names

Inosine phosphorylase, PUNP

Gene Symbol

PNP

Additional Purine Nucleoside Phosphorylase/PNP Products

Product Documents for Purine Nucleoside Phosphorylase/PNP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Purine Nucleoside Phosphorylase/PNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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